Abstract
Human iPSC-derived cardiomyocytes (hiPSC-CMs) are expected to be used in regenerative therapies and drug discovery for heart failure. hiPSC-CMs are a mixture of mainly ventricular CMs (VCMs) and also of atrial CMs (ACMs) and pacemaker cells. Here we describe a method to enrich VCM and ACM differentiation and to characterize these subtypes by gene expression analysis using qRT-PCR and by electrophysiological properties using the patch-clamp method. The differentiated VCMs and ACMs highly express VCM and ACM marker genes, respectively. Furthermore, both subtypes show specific properties of action potentials.
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Acknowledgments
We thank Peter Karagiannis for proof-reading the manuscript. This work was supported by a grant from Takeda Pharmaceutical Company Limited, a grant from Leducq foundation (18CVD05), JSPS KAKENHI Grants (19K16041 and 17H04176), grants from the Research Center Network for Realization of Regenerative Medicine (JP19bm0104001, JP19bm0204003, and JP19bm0804008), Research on Regulatory Science of Pharmaceuticals and Medical Devices (JP19mk0104117), and Research Project for Practical Applications of Regenerative Medicine (JP19bk0104095) provided by the Japan Agency for Medical Research and Development, and iPS research fund.
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Nakanishi-Koakutsu, M., Takaki, T., Miki, K., Yoshida, Y. (2021). Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human-Induced Pluripotent Stem Cells. In: Yoshida, Y. (eds) Pluripotent Stem-Cell Derived Cardiomyocytes. Methods in Molecular Biology, vol 2320. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1484-6_14
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DOI: https://doi.org/10.1007/978-1-0716-1484-6_14
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